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KMID : 0545119980080030208
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 3 p.208 ~ p.213
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Production of Cellulase by Trichoderma reesei Rut C30 in Wheat Bran-containing Media
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Yu, Xiao Bin
Yun, Hyun Shik/Koo, Yoon Mo
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Abstract
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The effect of the addition of wheat bran to the growth medium on the production of cellulolytic enzymes of Trichoderma reesei Rut C30 was studied in batch culture using shake flasks. The activity of cellulase was enhanced by the addition of wheat bran to the cellulase production medium. KH_2PO_4-K_2HPO_4 buffer was used for pH control during cellulase production. As a result, high cellulase activities were obtained in shake flask culture; a CMC (carboxymethyl cellulose) activity of 125.78 U/㎖ was obtained from 2% Avicel- and 3% wheat brancontaining medium and an FP (filter paper) activity of 12.85 U/㎖ was obtained from 1% Avicel- and 5% wheat brancontaining medium after 6 days of cultivation.
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